A spokeswoman for the Department of Foreign Affairs says Canadian officials in Mexico are providing consular assistance to the unidentified woman and her family.
"Our thoughts are with the badly injured Canadian and her family during this difficult time," said Aliya Mawani.
Mawani added that Canadian officials are also in contact with Mexican authorities.
Mexican news site noroeste.com reports a Canadian woman in her mid 20s was found in a pool of blood in an elevator at the upscale Hotel Riu Emerald Bay in the resort town of Mazatlan, which is on the country's west coast.
The media outlet says the woman was taken to two private clinics which refused to treat her before being taken to a hospital where she is in serious condition.
Noroeste.com also reports Mexican police saying it appears the woman might have been assaulted in her room and then dragged down a hallway — where traces of blood were found — to the elevator.
Canada's official travel warning for Mexico includes Mazatlan as an area which has experienced a surge in violence related to organized crime.
The incident is the latest report of violence involving Canadians visiting Mexico in recent weeks.
The body of Ximena Osequeda, a Vancouver resident and University of British Columbia student, was found last month partially buried on a beach in Huatulco, south of Acapulco.
Meanwhile, Robin Wood, 67, of Salt Spring Island, B.C., was killed in January when he stood up to two suspects who tried to rob a friend's home in the town of Melaque, south of Puerto Vallarta.Suggest a correction