Any resident of the province 18 or older can register to become a donor at www.signupforlife.ca
The information will be stored in a secure government database, which can be accessed quickly by doctors.
Manitobans will continue to have other ways of registering — by signing their health cards or the blue donor cards distributed by Manitoba Public Insurance.
The new registry website also gives donors the chance to help promote organ donation, through an optional link to their social media accounts on services such as Facebook.
The province first announced the registry a year ago at a cost of $100,000.