Via spokesperson Elizabeth Huart said that the collision involved Train 65 that was en route from Montreal to Toronto.
The incident took place on a section of track between Belleville and Cobourg, just after 8 p.m. ET.
Police, paramedics and firefighters attended the scene, as did a coroner.
It was not immediately clear when the train will be cleared to move. Some trains were moving past the scene of the collision.