Gérald Tremblay refused to comment on the allegations that his party got a cut from from a bid-rigging operation involving public infrastructure contracts.
That testimony, from former construction mogul Lino Zambito, resulted in the suspension of three city employees and triggered further internal investigations at City Hall.
It has also reignited calls for Tremblay's resignation.
Both opposition parties at City Hall are set to file motions at the next council meeting, on Oct. 22, asking for Tremblay to step down.
On Tuesday, the president of the Montreal Police Brotherhood said allegations of corruption that have surfaced during the inquiry into the construction industry have tainted his association's view of the mayor.
He asked the provincial government to step in and strip the Tremblay administration of its power to set police budgets and priorities.
The mayor has denied the allegations and said his party's finances have been verified by Quebec's chief electoral officer without any findings of wrongdoing. He has said he has no intention of stepping down.
On Wednesday, Tremblay said he has long called for a public hearing on corruption allegations and he would welcome the oppourtunity to be called as a witness.
"I have full confidence in the commission," he said. "I would definitely strongly recommend the commission ask me to testify."
The Charbonneau commission is on a break this week. Testimony will resume Monday.