Guillermo Ramirez and Gustavo Cabrera both played 100 times for Guatemala, including in 2014 World Cup qualifiers, and had captained the team.
FIFA said Wednesday that Ramirez, Cabrera and a third national team player, Yoni Flores, are "banned from taking part in any kind of football-related activity, for life."
The players were involved in fixing Guatemala's 2-0 home defeat against Venezuela in June 2011 and a 3-2 loss in Costa Rica in May 2012.
FIFA said the charges also included a CONCACAF Champions League group-stage match between Guatemalan club CSD Municipal and Santos Laguna of Mexico on Oct. 19, 2010. Santos won 6-1.
FIFA has extended the bans globally after they were imposed nationally last month by the Guatemala Football Association.
Ramirez is a 34-year-old midfielder, Cabrera is a 32-year-old defender and Flores is a 29-year-old defender.
FIFA did not detail how the players were involved in fixing matches.
Earlier in the investigation, FIFA also studied a fourth suspicious match — Guatemala's 5-0 defeat against 2010 World Cup host South Africa in a friendly played in Polokwane two weeks before the tournament kicked off.
That match was refereed by Niger official Ibrahim Chaibou who has been linked with several match-fixing probes in which goals were scored from penalties for dubious handball offences. FIFA investigators have been unable to meet Chaibou for questioning.