The regulation applies to Canadians or permanent residents who bring their spouse to Canada from overseas.
They'll now have to live together in what the government calls a legitimate relationship for two years or the sponsored spouse could lose permanent resident status.
The rule will only apply to those who have been married less than two years and have no children together at the time of their application.
Immigration Minister Jason Kenney says sometimes the sponsor in Canada is being duped and sometimes the marriage is actually a commercial transaction.
He says implementing a two-year conditional permanent residence period will help deter fraud and the victimization of Canadians.
The conditional status will be waived in cases where there is evidence of abuse or neglect or where the spouse already in Canada dies.
The new regulation will apply to all applications received after today.
It follows measures introduced earlier this year also aimed at sponsored spouses.
Those rules require a sponsored spouse to be a permanent resident for five years before they in turn could bring a partner or spouse to Canada.
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