In Nova Scotia, about 20 people from the Millbrook First Nation placed wooden palettes and a car on the tracks in Truro at about 1 p.m.
Chief Bob Gloade said the blockade of passenger and freight trains that travel through the reserve's land was planned until 5 p.m., but the barricade was left in place because protest leaders were waiting to learn what happened at a crucial meeting in Ottawa between Prime Minister Stephen Harper and First Nations leaders.
A spokesman for the Nova Scotia protesters said the group had been speaking with officials at CN and Via Rail, but there was no indication any trains would be allowed to pass.
Gloade said the blockade was sanctioned by the band council and is part of a growing wave of protests and blockades organized by the Idle No More movement.
Via Rail said in an email that it took 53 passengers to Truro from Halifax by bus.
Spokeswoman Mylene Belanger said the next passenger train scheduled to roll through the area is expected in Truro on Saturday at 3:47 p.m. She said any negotiations with the protesters would be conducted by the owners of the tracks, CN.
In New Brunswick, about 250 people — most of them members of the Listuguj First Nation — marched across the bridge that links Cambellton, N.B., with Pointe-a-la-Croix, Que.
Campbellton Mayor Bruce MacIntosh said the bridge was closed between 2 p.m. and 3 p.m. as protesters marched with banners from the Quebec to New Brunswick and back again.
"We knew what was going to take place in the last couple of days," MacIntosh said in an interview from his home. "We were in place with the RCMP to deal with it accordingly ... Everything is moving well know."