The Monday morning service remembered 40-year-old Alison (Constance) Easton and her two children,10-year-old Alex Corchis and six-year-old Katie Corchis.
Police believe Easton killed her son and daughter before taking her own life in Stittsville on Jan. 14.
No details about cause of death have been released, but the autopsies were conducted last week.
The deaths have rocked the west Ottawa community where both children attended Stittsville Public School just across the street from their home.
Easton was often seen walking her children to and from school, according to a neighbour, where the girl was in kindergarten and the son was in Grade 5.
Police also said the mother was involved in the local neighbourhood watch program.
Easton and her husband, Jon Corchis, were married for 15 years. The funeral took place at All Saints Anglican Church in Windsor because both parents and their families are from the southern Ontario city.