The couple is to face a two-week preliminary hearing in June.
Paramedics were called last May to a home where they found a two-year-old girl and her sister suffering from injuries and severe malnourishment.
One girl died shortly after the Supreme Court of Canada refused to interfere with a lower court ruling that doctors be allowed to remove the child from life support.
The Crown laid new charges against the parents this week: second-degree murder and criminal negligence causing death.
The still face original charges of aggravated assault, criminal negligence causing bodily harm and failing to provide the necessities of life.
Also on HuffPost