The closure will begin at 2 a.m. ET on Saturday morning and affect both northbound and southbound lanes between the Gardiner Expressway and Highway 401.
"We hope people understand the importance of the work we're doing so that the roads are in good working order for them all the other days of the year," said Myles Currie with the city's transportation services.
City crews will be repairing potholes, cleaning catch basins and doing maintenance on signs and road markings.
A water main pipe will also be installed at the Eastern Bridge, near Eastern Avenue and Broadview Avenue.
It's enough for Markham's Brian Ruddock to change his weekend travel plans.
"I will not be coming down to the city. There's too much congestion on the weekends and especially now fixing all the roads and everything," he said.
The DVP will reopen again on Monday at 5 a.m. ET.