The incident happened this morning near Glen Robertson and Massie Roads, OPP confirmed. Alexandria is about 100 km east of Ottawa, according to Ontario Provincial Police.
The collision happened at 7:30 a.m., said Via spokeswoman Mylene Belanger.
Via said the route between Ottawa and Montreal is now clear and all train travel will resume as planned.
The train had left Ottawa at 6 a.m. and was bound for Montreal at 8:30 a.m. There are 150 passengers on the train, Via confirmed.
OPP investigators are calling it a "non-suspicious, sudden death".
VIA said the person was a pedestrian.
The train has been waiting outside Alexandria for more than two hours since the incident, according to one passenger aboard the train.
Ontario Provincial Police are investigating.