The Acadian Federation of Nova Scotia says it filed a notice of application against the province in Yarmouth provincial court.
Federation president Justin Mury says the group believes the changes violate the principle of effective representation as defined by the Supreme Court of Canada.
Justice Minister Ross Landry says he respects the group's right to pursue court action, but maintains the new map is legitimate.
In April, Premier Darrell Dexter refused a request by the federation to have the Nova Scotia Court of Appeal rule on the constitutionality of riding changes.
The new boundaries, passed in the legislature in December, merged three ridings intended to represent Acadian populations with other ridings