The 5 kilometre race and 3 kilometre walk in downtown Toronto raises money for local charities and has become a popular part of the city's Pride Festival.
This year Wynne, Canada's first openly gay premier, laced on her running shoes and sprinted the route alongside about 1,000 others.
Afterwards she spoke to the crowd, telling them a lot has been accomplished in the past decade when it comes to gay rights, but those gains shouldn't be taken for granted.
"We have come a long way, you know, 10 years since same sex marriage in this country was legalized," she said. "But the fact is that our rights and our privileges are precious and we have to guard them."
The premier had a very respectable final time of 26:21, which placed her ninth out of 38 women in her age group.