All three of the chicks in the nest near the Whitehorse waterfront have fledged, and all three were out of the nest at the same time on Tuesday morning.
The eagles have become Internet celebrities this summer, as the territory's power utility set up a webcam to watch their development earlier this spring.
The website became very popular online, drawing almost a million views since being set up in May, Yukon Electric spokesperson Laura Carlson told CBC News Tuesday.
Thousands of people, many of whom are from nowhere near Yukon, have become regular viewers. "We have gotten to know some of our chatters some are from Nebraska and Virginia," Carlson said. "Some are local chatters, some are from Nova Scotia, so it's really become a little community unto itself of Yukon eagles."
"It's kind of heartwarming to see these people brought together watching our eagles grow up," she said.
Carlson says the power utility will leave the eagle cam operating at least another week to monitor continued use of the nest. The camera was off briefly on Tuesday because it's been overheating.
But biologists expect the nest will remain home base for another week or so before it's abandoned for good.