Vincent-Lapointe, from Trois-Rivieres, Que., finished in 51.330 seconds for the victory.
"I didn't have a great start but I charged it and kept going strong to the finish," said Vincent-Lapointe. "In the end I finished with the best possible result."
Vincent-Lapointe has won gold on four other occasions at the senior world championships — twice in 2010 and twice in 2011. She'll go for another gold tomorrow in the C2 500 final with Sarah-Jane Caumartin of Otterburn, Que.
Ben Russell of Dartmouth, N.S., and Gabriel Beauchesne-Sevigny of Trois-Rivieres, Que., finished fourth in the C2 1000-metre final, missing out on a medal by less than a quarter of a second.
"We have mixed emotions about just missing the podium but we had the fastest last 250 and good travel speed throughout the whole race," said Russell.
It was Canada's best result in the event since Tamas and Attila Buday won a silver medal in 2006.
Mark Oldershaw of Burlington, Ont., was a disappointing ninth in the men's C1 1000 final. He won a bronze medal in the event at the London Games last year.
Halifax's Mark de Jonge, who won Olympic bronze last year, won his semifinal in the K1 200 metres to qualify for Sunday's final. Ryan Cochrane of Windsor, N.S., and Richard Dober Jr., of Trois-Rivieres, Que., also advanced by finishing third in the K2 200 semifinal.
Jason McCoombs of Dartmouth, N.S., finished fourth in the men's C1 200-metre semifinal and did not advance to the final.
Michelle Russell of Waverley, N.S., also came up short after finishing fifth in the women's K1 200-metre semifinal while Paul Bryant and Roland Varga of Richmond Hill, Ont., settled for a seventh-place finish in the C2 200 metre semifinal.