Eron Lamar Riley, 26, appeared briefly in Regina provincial court Friday morning on a charge of aggravated assault.
Two other players with the Regina-based Canadian Football League team — Dwight Anderson and Taj Smith — were arrested earlier this week and appeared in court Thursday facing the same Criminal Code charge.
After a night in jail, Anderson and Smith were released pending their next court appearance Oct. 8.
Riley was released Friday morning and will also be back in court in October.
The charges stem from a physical altercation that happened outside a nightclub on the 2200 block of Dewdney Ave. in the city's warehouse district on Aug. 18.
A man, 20, was found seriously injured. He was taken to hospital for treatment.
The Roughriders have not suspended Anderson or Smith, and will announce today whether or not they will play in the team's next game, which is against Toronto at home on Saturday.
Under the terms of their release from custody, Anderson, Smith and Riley must abide by a number of conditions, including avoiding alcohol and drugs, and staying away from nightclubs.
The lawyer for Smith and Anderson said there is no restriction that would prevent them from travelling with the team.
There was no immediate response from the Roughriders on Riley, a wide receiver who is in his first season with the team.