J.D. Martinez took a called third strike from New York Yankees reliever Dellin Betances that ended the ninth inning Sunday, giving Houston 14 strikeouts for the game and 1,530 for the season.
Arizona held the previous mark of 1,529 in 2010.
Betances struck out the side in the ninth. New York starter David Huff had seven strikeouts in five innings, and reliever Brett Marshall three.
Houston's Chris Carter struck out three times in his first four plate appearances, raising his season total to 212. Only Mark Reynolds (223 in 2009) and Adam Dunn (222 last year) have struck out more.