Friends and family of Nicole Hannah Whiteduck and Laura Spence say the women were last seen Sunday morning on the reserve in Maniwaki, Que., located about 130 kilometres north of Ottawa.
Whiteduck and Spence could have been en route to Ottawa, according to a missing poster circulating online. It also says their money, wallets and cellphones were left at home.
Both women have brown hair and Whiteduck has a tattoo on her left shoulder.
The disappearance of Whiteduck and Spence comes a week after James Anaya, the UN special rapporteur on the rights of indigenous people, called on the federal government to launch a "comprehensive and nationwide" inquiry into the case of missing and murdered aboriginal women.
This summer, Canada's provinces also issued a joint call for a full inquiry. The federal NDP and Liberals have also requested this, but the government has yet to agree.
The Native Women's Association of Canada estimates there are more than 600 missing and murdered First Nations women across the country.
Two aboriginal women — Shannon Alexander and Maisy Odjick — went missing from Maniwaki more than five years ago. They have not been heard from since. Montreal Police say their investigation into their disappearance remains open.
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