NBCU had a 47 per cent stake previously while remaining partners PBS had about 17 per cent and Hit had about 35 per cent. Sesame Workshop had sold its stake prior to Wednesday's announcement.
Terms of the deal were not disclosed.
PBS' brand will slowly be removed from the network, previously known as PBS Kids Sprout, over the next few months, although popular PBS programs such as "Sesame Street" and "Caillou" will still be shown. The 24-hour network reaches more than 60 million homes in the U.S. and competes with channels like Nick Jr. and Disney Junior.