At a meeting last night, the English Montreal School Board (EMSB) council of commissioners passed the resolution, which refuses to carry out the secular charter's key provisions.
"[Bill 60] runs contrary to what we as educators try to teach our kids," said EMSB commissioner Syd Wise.
"Our teachers teach tolerance of others, respect and recognition of individual rights, which includes freedom to adhere to one's religious convictions."
The Parti Québécois's controversial charter would ban employees in the public sector, such as teachers and doctors, from wearing overt religious symbols including the hijab, the yarmulke and large crucifixes.
The school board says it has no intention of complying with those provisions, which it says runs contrary to the school board's core values.
The EMSB is expected to present a brief to Quebec's national assembly in early 2014.