The device known as Google Glass, not yet widely available to the public, features a thumbnail-size transparent display above the right eye.
Cecilia Abadie was pulled over on suspicion of speeding in October. The officer saw she had Google Glass and tacked on a citation usually given to drivers who may be distracted by a video screen.
On Tuesday, Abadie pleaded not guilty to both charges in San Diego traffic court.
Her lawyer says she will testify at a trial scheduled for January that the glasses were not on when Abadie was driving, but activated when she looked up at the officer.