Alltheway Trucking Inc. and the driver were each found guilty under the Fisheries Act for possessing live invasive fish.
The company was fined $70,000 and the truck driver was fined $5,000.
Officials seized more than 3,000 kg of grass carp.
The fine comes nearly two years after a pair of incidents in which the company and driver were charged in Windsor, Ont..
Court heard that on Jan. 25, a truck from Alltheway Trucking Inc. entered Canada at the Ambassador Bridge in Windsor, carrying grass and bighead carp from the state of Arkansas.
The Canada Border Services Agency contacted the Ministry of Natural Resources to assist with the inspection of the truck.
Conservation officers with the Lake Erie enforcement unit inspected the truck and found some of the grass carp were still alive.
They subsequently seized all of the fish.
On Feb. 28, 2012, the same truck and driver again entered Canada at the Ambassador Bridge carrying bighead carp from the state of Arkansas.
After being contacted by the CBSA, conservation officers found 6,350 kg of bighead carp, some of which were alive.
They seized the truck and fish.
Both incidences resulted in charges.
It has been illegal to possess live invasive fish, including bighead, grass, black and silver carp in Ontario since 2005 because of the significant threat they pose to the province’s lake systems.