An Amber Alert remained in effect across Alberta on Wednesday as police continue to search for leads in the perplexing disappearance of a five-year-old boy and his grandparents.

Nathan O'Brien was reported missing Monday morning when his mother went to pick him up after a sleepover at the grandparents' southwest Calgary home and they weren't there.

Police say based on things they found in the home, they don't believe the residents left voluntarily.

Also missing are O'Brien's grandparents, 66-year-old Alvin Cecil Liknes and Kathryn Faye Liknes, 53, who are not suspects.

Nathan's father, Rod O'Brien, made an emotional appeal Wednesday that was carried live on national TV.

"Nathan, we are going to bring you home very soon,'' O'Brien said. "Nathan, God is taking care of your soul and Mom and Dad need you to be brave right now.

"Whoever has Nathan, please find it in your heart to drop off our little angel Nathan (so) he can be rescued and reunited with his brothers and his family.''

Investigators are asking anyone who attended an estate sale at the residence in the 100 block of 38A Avenue S.W. over the weekend to visit the Parkhill Community Centre on Thursday, July 3, between 10 a.m. and 10 p.m.

Those who purchased something are asked to bring a photo of that item to help detectives determine what items were bought during the sale, compared to what items remain in the home.

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  • Mourners have placed flowers, notes and teddy bears at an impromptu memorial for Nathan O'Brien and his grandparents, Alvin and Kathryn Liknes, at the Liknes home in Calgary, Alta., Tuesday, July 15, 2014.

  • Flowers and teddy bears are shown at an impromptu memorial for Nathan O'Brien and his grandparents, Alvin and Kathryn Liknes, at the Liknes home in Calgary, Alta., Tuesday, July 15, 2014. A man who has been under investigation in the disappearance of a Calgary boy and his grandparents has been formally charged with their murders.

  • Bobbi-Jo Zimmer, from High River, Alta., and her children, left to right, Tayvon, two, Braylon, seven, and Dawson, nine, place flowers at an impromptu memorial for Nathan O'Brien and his grandparents, Alvin and Kathryn Liknes, at the Liknes home in Calgary, Alta., Tuesday, July 15, 2014.

  • Mourners have placed flowers, notes and teddy bears at an impromptu memorial for Nathan O'Brien and his grandparents, Alvin and Kathryn Liknes, at the Liknes home in Calgary, Alta., Tuesday, July 15, 2014.

  • Kelley MacAuley observes a memorial after placing flowers at an impromptu memorial for Nathan O'Brien and his grandparents, Alvin and Kathryn Liknes, at the Liknes home in Calgary, Alta., Tuesday, July 15, 2014.

  • Jennifer Kohut, centre, embraces her sons Axton, left, three, and Cadon, five, after placing flowers and toys at an impromptu memorial for Nathan O'Brien and his grandparents, Alvin and Kathryn Liknes, at the Liknes home in Calgary, Alta., Tuesday, July 15, 2014.

  • Douglas Garland is escorted into a Calgary police station in connection with the disappearance of Nathan O'Brien and his grandparents in Calgary, Alta., Monday, July 14, 2014.

  • Douglas Garland is escorted into a Calgary police station in connection with the disappearance of Nathan O'Brien and his grandparents in Calgary, Alta., Monday, July 14, 2014.

  • Douglas Garland is escorted into a Calgary police station in connection with the disappearance of Nathan O'Brien and his grandparents in Calgary, Alta., Monday, July 14, 2014.

  • Douglas Garland is escorted into a Calgary police station in connection with the disappearance of Nathan O'Brien and his grandparents in Calgary, Alta., Monday, July 14, 2014.

  • Calgary Police Chief Rick Hanson says first and second degree murder charges are being filed in connection with the disappearance of Nathan O'Brien and his grandparents at a news conference in Calgary, Alta., Monday, July 14, 2014.

  • Calgary Police Chief Rick Hanson says first and second degree murder charges are being filed in connection with the disappearance of Nathan O'Brien and his grandparents at a news conference in Calgary, Alta., Monday, July 14, 2014.

  • Calgary Police Chief Rick Hanson says first and second degree murder charges are being filed in connection with the disappearance of Nathan O'Brien and his grandparents at a news conference in Calgary, Alta., Monday, July 14, 2014.

  • People comfort each other at a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • People comfort each other at a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Volunteers prepare candles at a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • A photo collage of the Liknes and O'Brien family is displayed as neighbors, friends and relatives hold a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Rod O'Brien, centre, tears up as he looks at a photo collage of the Liknes and O'Brien family during a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • A photo collage of the Liknes and O'Brien family is displayed as neighbors, friends and relatives hold a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Rod O'Brien, centre, father of Nathan O'Brien, attends with neighbors, friends and relatives, a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Jennifer O'Brien, left, mother of Nathan O'Brien, attends with neighbors, friends and relatives, a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Rod O'Brien, and Jennifer O'Brien attend a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Neighbors, friends and relatives hold a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Rod O'Brien, right, and Jennifer O'Brien, centre, attend a candlelight vigil for missing Calgarians Nathan O'Brien, five, and his grandparents Alvin and Kathryn Liknes in Calgary, Alta., Thursday, July 10, 2014.

  • Police investigators comb a hay field north of Airdrie, Alta., Monday, July 7, 2014, looking for clues to the disappearance of three people.

  • Police investigators use an inflatable raft in a slough north of Airdrie, Alta., Monday, July 7, 2014, looking for clues to the disappearance of three people.

  • Police investigators prepare to search a hay field north of Airdrie, Alta., Monday, July 7, 2014, looking for clues to the disappearance of three people.

  • Police investigators prepare to search a hay field north of Airdrie, Alta., Monday, July 7, 2014, looking for clues to the disappearance of three people.

  • Police investigators prepare to search a hay field north of Airdrie, Alta., Monday, July 7, 2014, looking for clues to the disappearance of three people.

  • Police investigators prepare to search a hay field north of Airdrie, Alta., Monday, July 7, 2014, looking for clues to the disappearance of three people.

  • Police investigators at a rural residence north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators comb a hay field north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators comb a hay field north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators comb a hay field north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators comb a hay field north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators comb a hay field north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators at a rural residence north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Police investigators at a rural residence north of Airdrie, Alta., Saturday, July 5, 2014, looking for clues to the disappearance of three people.

  • Rod O'Brien wipes his tears while holding a butterfly made by his son as he speaks to the media about his son Nathan O'Brien and his grandparents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Rod and Jennifer O'Brien show their emotions as they speak to the media about their son Nathan O'Brien and his grandparents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Rod and Jennifer O'Brien left, look on as Jeff Liknes, second from right, speaks to the media about his nephew Nathan O'Brien and his parents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Jennifer O'Brien holds a piece of her son's clothing while speaking to the media about her son Nathan O'Brien and his grandparents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Jennifer O'Brien holds a piece of her son's clothing while speaking to the media about her son Nathan O'Brien and his grandparents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Rod O'Brien wipes his tears while holding a butterfly made by his son as he speaks to the media about his son Nathan O'Brien and his grandparents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Rod and Jennifer O'Brien show their emotions as they speak to the media about their son Nathan O'Brien and his grandparents Alvin and Kathryn Liknes during a press conference in Calgary on Wednesday, July 2, 2014.

  • Nathan O'Brien is described as three feet tall, curly blonde hair, brown eyes, and was last seen wearing peach-coloured shorts and a blue-striped hoodie.

  • Kathryn Liknes is five-foot-three-inches tall, 120 pounds with reddish-brown hair and green eyes.

  • Alvin Liknes is six feet tall, 200 pounds with grey and blond hair and was wearing black shorts. Alvin has a distinctive walk.

  • Calgary police investigators check out the home where five-year-old Nathan O'Brien and his grandparents Alvin and Kathryn Liknes disappeared, in Calgary, Alta., Wednesday, July 2, 2014.

  • Nathan O'Brien and his grandmother Kathy Liknes are shown in a Calgary Police Service handout photo. Calgary's police chief questions whether a reward would help the investigation into the disappearance of a five-year-old boy and his grandparents.

  • Police tape cordons off the home where five-year-old Nathan O'Brien and his grandparents Alvin and Kathryn Liknes disappeared, in Calgary, Alta., Wednesday, July 2, 2014.

According to police, officers in the Liknes' neighbourhood have interviewed neighbours, family members and friends of the family, and also performed a grid search of the area, but have no significant leads.

"We have a big mystery on our hands right now. We don't know where these people are. We are still very optimistic that they are alive, and we are doing everything we can to locate them, but we need the help of the public," Duty Insp. Keith Cain told reporters Tuesday afternoon.

Calgary police spokesman Kevin Brookwell told CTV News there are no custody issues with the family and that all of the family's vehicles are accounted for.

Nathan’s father, Rod O’Brien, told the Calgary Sun a reward consisting of a “large sum” would be announced Wednesday.

According to CTV News, the reward could be in excess of $100,000.

Nathan O'Brien is described as three feet tall, curly blonde hair, brown eyes, and was last seen wearing peach-coloured shorts and a blue-striped hoodie.

nathan obrien

Alvin Liknes is six feet tall, 200 pounds with grey and blond hair and was wearing black shorts. Alvin has a distinctive walk.

alvin liknes

Kathryn Liknes is five-foot-three-inches tall, 120 pounds with reddish-brown hair and green eyes.

kathryn liknes

Anyone with information about the case should phone police at 403-266-1234 or call Crime Stoppers.

With files from the Canadian Press