The Supreme Court of Canada dismissed the request on Friday morning, with costs.
Accurso faces criminal charges in several municipal corruption cases. He's also charged with alleged tax fraud.
He has argued that if he is forced to testify before the Charbonneau commission, he risks incriminating himself and prejudicing his criminal trial.
In a previous ruling, a Quebec Superior Court justice explained there are already guarantees in place to avoid that from happening.
Commissioners have promised not to question Accurso about his pending criminal proceedings.
Accurso is set to appear before the Charbonneau commission during the first week of September.
The commission is mandated with investigating corruption and collusion in the Quebec construction industry.
More to come