The sloth underwent an ultrasound this week, a video of which was released on YouTube.
She is one of four female sloths at the Biodome and she herself was born in the facility.
The Biodome does not name its animals, but this particular sloth became well-known to the staff after she, at eight-months-old, fell and broke her femur inside the Tropical Rainforest environment. She underwent surgery at the Université de Montréal Faculty of Veterinary Medicine and had metal rods inserted in her leg to stabilize the bone.
She recovered at the Biodome's veterinary clinic and rejoined the other sloths after 10 months of rehabilitation.
Since 2002, eight sloths have been born at the Biodome. Five of them have been transferred to other zoos.
Few two-toed sloths births have been documented, according to the Biodome, but the gestation period is around 11 months.
The Biodome expects the newest sloth to arrive this fall.