The decision was announced Monday in a Tennis Canada release. Prospect Charlotte Robillard-Millette of Blainville, Que., will fill the final roster spot after the world's seventh-ranked player decided she would not compete in the first-round tie.
"Of course we are disappointed that Genie is unable to be available for this tie and we know her fans in Quebec City will be disappointed as well," Tennis Canada president Kelly Murumets said in a statement. "However, Genie wants to be the best in the world and win Grand Slams and she needs to make the right decisions for her schedule and her training to give her the best opportunity to achieve that.
"Tennis Canada is fully supportive of Genie as she strives towards her goals."
Bouchard, from Westmount, Que., reached the quarter-finals at the recent Australian Open before losing to Russia's Maria Sharapova. After the match, Bouchard said she had yet to make a decision on the Fed Cup, adding she planned to visit with family and take some time off to rest.
Tennis Canada named three members of its Fed Cup team last week but left the remaining spot open as it waited for Bouchard's decision. The association had until Friday to finalize its roster.
Bouchard's absence leaves little star power in the best-of-five series. The Czechs will be without their top two players, as world No. 4 Petra Kvitova and No. 15 Lucie Safarova are not on their team roster.
The 16-year-old Robillard-Millette joins Montreal's Francoise Abanda, Ottawa's Gabriela Dabrowski and Toronto's Sharon Fichman on the team.
Bouchard won all four of her matches last year to help Canada reach the elite World Group I for the first time. She is entered in an indoor tournament in Belgium next week.
Team captain Sylvain Bruneau said he thinks Bouchard will play again for Canada in the future, adding he understands that busy schedules often impact top players.
"She's no different than any other player in the top 10 on the female or the male side," he said on a conference call. "They don't always play Fed Cup every single time that they are asked — or Davis Cup. It's different. She's top 10, she wants to be winning Grand Slams and I think she's going to be making decisions every single time if it's good or not and I get it."
Bouchard's agent informed Murumets of the decision, Bruneau said.
Bouchard was not available for comment. A message left with her agent was not immediately returned.
The top-ranked Czechs will be heavy favourites against the eighth-ranked host side. The Czech roster includes 22nd-ranked Karolina Pliskova, Tereza Smitkova (No. 62), Denisa Allertova (No. 91), and Lucie Hradecka (No. 122).
Fichman is the top-ranked Canadian for the tie at No. 135. Dabrowski is next at No. 185 while Abanda holds the No. 230 position. Robillard-Millette is No. 57 on the ITF junior rankings list and No. 814 on the WTA list.
Aleksandra Wozniak of Blainville, Que., was on the Canadian team last year but she's still recovering from shoulder surgery.
The Czechs have been crowned Fed Cup champions three of the last four years.