OPP Sgt. Kerry Schmidt said the driver of a fully-loaded tractor-trailer was killed when the truck collided with a Jeep and flipped over near the Brock Street exit just after 10 p.m.
The driver, identified as 51-year-old Lindsay A. Findlay of Arnprior, Ont., was pronounced dead at the scene.
Schmidt said the driver of the Jeep had lost control of his vehicle and was hit from behind by transport, which rolled over and blocked all three lanes of the highway.
The highway has been blocked off throughout the night, with some drivers stuck for up to four hours as police called in heavy equipment to assist in the clean up.
The transport truck was filled with grain, said Schmidt, which was causing problems for crews trying to clear the highway of obstructions.
The Jeep driver, a 32-year-old North York man, was not injured but was arrested and charged with impaired driving causing death.
Police have not indicated when the highway will re-open, but said they hope to have the lanes cleared before rush hour begins.