Jennifer Clark, who is 38, and Jeromie Clark, who is 34, were charged in December with criminal negligence causing death and failure to provide the necessities of life.
Their 14-month-old son, John, died the day after he was brought to a Calgary hospital in November 2013.
Police have said the child had a staph infection that was untreatable due to complications from malnutrition.
They said the family has strict dietary restrictions based on their faith and nutritional beliefs.
The three-day hearing, which will determine if there is enough evidence to proceed to trial, is scheduled for Nov. 2.