Wallenda said Monday at a New York City news conference that the Orlando Eye will be moving when he attempts his feat April 29.
The attraction in the heart of Orlando's tourist district officially opens in May.
The Orlando Eye team issued a statement saying it's excited to have Wallenda attempt the "amazing stunt."
In November, Wallenda made two Chicago skyscraper crossings on high wires without a safety net or a harness.
Previous tightrope walks took him over the brink of Niagara Falls in 2012 and across a Grand Canyon-area gorge in 2013.
Wallenda is the great-grandson of Karl Wallenda, who fell to his death in Puerto Rico at 73.
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