Jacoby confronted the umpire crew following the Blue Jays' 4-1 in Boston on April 29.
The 55-year-old was unapologetic in a statement released by the club after his appeal was denied.
"Unfortunately, there was a verbal altercation with the umpiring crew following a tough loss in Boston," he said. "Frustrations escalated, leading to an altercation in which I was wrongly accused of contacting an umpire in the runway following our game, I'm in no way going to apologize for what happened and feel that the penalties were very biased, harsh, and unfair.
"I feel vindicated by the fact that everyone very near to the incident corroborated my actions when interviewed."
Jacoby said he will not comment on further on the incident.