The demonstration coincides with the beginning of a three-day hearing on the city's request to get a permanent injunction to halt Uber's operations in Toronto. Similar injunctions are in place in some other Canadian cities like Vancouver and Calgary, though others have embraced Uber as an inevitability of modern technology.
The protest is set to begin near the intersection of Yonge Street and Queens Quay before moving north up Yonge to Queen's Park around 9 a.m. ET.
In recent months, drivers from various companies have staged similar protests against what they say is unfair standards for Uber, which does not pay the same fees to operate in the city or insurance costs as traditional taxi services.
Critics also argue that because Uber trips are not eligible under insurance plans that cover licensed taxi rides, the company's prominence puts the public at risk.
Uber doesn't own a fleet of vehicles, but instead has a service that connects customers to drivers who charge for the ride they offer. Uber co-ordinates the payments.
Mayor John Tory has repeatedly said he opposes the injunction and welcomes the new competition in an industry long dominated by just a few large companies.
Uber has maintained that they are simply an app that connects people to drivers willing to take them where they need to go.