Will Crothers, Tim Schrijver, Kai Langerfeld and Conlin McCabe won the final in six minutes 10.80 seconds, more than three seconds ahead of silver medallists Cuba.
Argentina won bronze.
Earlier on Monday, Kerry Shaffer and Antje Von Seydlitz won Canada's first rowing gold medal at the Pan American Games.
Shaffer, from nearby Welland, Ont., and Von Seydlitz, from Smithers, B.C., finished the women's double sculls in seven minutes 13.01 seconds.
The Canadians were more than a second ahead of the United States sin silver position.
Cuba was seven seconds back for the bronze.
Also, Canadians Pascal Lussier and Matthew Buie won bronze in the men's double sculls.
Lussier, from Napierville, Que., and Buie, from Duntroon, Ont. finished third behind Cuba and Argentina.
The Cubans won gold in 6:30.35. Argentina was three seconds behind in 6:33.59 and the Canadians crossed third in 6:35.89.