The Canadian trio of Hugo Barrette, Evan Carey and Joseph Veloce completed a comeback victory with a time of 44.241 seconds.
Venezuela's Hersony Canelon Vera, Cesar Marcano Sanchez and Angel Pulgar Araujo came up short, clocking in at 45.087.
Brazil rounded out the podium with the bronze.
The Canadian women's sprint team will also vy for gold at 6:53 p.m. ET.
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