Trinity College lost a squash match to Yale 5-4 on Wednesday, snapping a 252-match winning streak that dated back to the 1997-98 season.
The Bantams are the 13-time defending national champions and were 8-0 heading into the match.
Trinity officials had called this year's team the school's most inexperienced in years. The Bantams lost six starters and five All-Americans from last year's championship squad.
Yale, which lost to Trinity in the last two national championship matches, features three of the nation's top 25 players.
The Bulldogs improve to 7-0 this season.