A handful of demonstrators pitched tents Tuesday outside the provincial courthouse on Queen Street West to protest a controversial budget vote at nearby city hall.
The protesters have argued the city can't force them off provincial land.
But the province gave the protesters notice to leave by 5 p.m. Thursday.
"They have respectfully been requested to have the structures removed this afternoon," Infrastructure Ontario spokesman Bill Moore says in an email.
"We are monitoring the situation closely and anticipate a peaceful end to this demonstration."
The tents were still standing shortly after the deadline and it isn't clear what will happen if the protesters hold their ground.
City officials dismantled the group's original encampment at St. James Park in late November.
Protesters calling attention to what they viewed as economic and social inequalities occupied the park for 39 days before they were forced to leave.
(The Canadian Press, 680News)