The 37-year-old native of Kingston, Ont., said Thursday that will remain in Victoria to get back to full running capacity before the start of the Games.
"I was really looking forward to racing in front of the passionate triathlon fans in Edmonton, but unfortunately I just can't risk having it develop into anything worse this close to the Olympics," Whitfield said.
"I have had a small tweak in my hamstring during training. It is feeling good right now. I'm fortunate to have coach Jon Brown, and our super physio, Marilyn Adams, to lean on and guide me back to full training. I expect to be back at 100 per cent by early next week, but in this case caution is the better part of valour."
Kyle Jones, of Oakville, Ont., will be Canada's lone Olympic-bound athlete to compete in the World Cup this weekend.