Bolt cruised through the race in 20.18 seconds. South Africa's Anaso Jobodwana came in second with a time of 20.27 to qualify for the next round.
Joining Bolt in the final on Thursday are teammates Yohan Blake, who won his semifinal heat in 20.01, and Warren Weir, who reached the final with a time of 20.28.
Canada's Aaron Brown and Jared Connaughton did not advance. Brown recorded a personal best of 20.42, while Connaughton finished in 20.64.
U.S. sprinter Felix wins gold in women's 200m final
American Allyson Felix won the first individual Olympic gold of her career in the women's 200m final.
The 26-year-old ran a time of 21.88 seconds, edging two-time 100m champion Shelly-Ann Fraser-Pryce of Jamaica, who ran a personal best 22.09.
Carmelita Jeter of the U.S. took home silver with a time of 22.14.
Felix, a silver medalist in the 200 in 2004 and 2008, was behind coming off the turn but powered past Fraser-Pryce over the last 40 metres.
Americans Merritt, Richardson claim gold, silver in men's 110m hurdles
Aries Merritt led a 1-2 finish for the United States in the Olympic 110m hurdles, winning in 12.92 seconds Wednesday night. Defending champion Dayron Robles of Cuba pulled up midway through and clutched his right hamstring.
Jason Richardson, the world champion, was second in 13.04. Hansle Parchment of Jamaica took the bronze in 13.12.
Robles, the world-record holder, began limping early and came to a stop after clearing the sixth hurdle, then shoved another barrier down to the track.
The 2004 Olympic champion, Liu Xiang of China, got injured in the first round of qualifying and stopped before reaching the first hurdle.
U.S. earns gold, bronze in long jump final
Brittney Reese won the long jump at the London Games on Wednesday night, becoming only the second U.S. woman to win the event at the Olympics.
The two-time world champion went into the competition with the best jump of the season and put down the best mark in the final with 7.12 metres on her second attempt. She is first U.S. woman to win the Olympic long jump since Jackie Joyner-Kersee in 1988.
Russia's Elena Sokolova took the silver medal with a best jump of 7.07 metres, and Janay Deloach earned the bronze for the United States at 6.89.
Russia's Antyukh hangs on for gold in 400m hurdles
Russia's Natalya Antyukh held off American Lashinda Demus to claim gold in the women's 400m hurdles final.
Antyukh ran a personal-best time of 52.70 seconds. Demus had a strong finish but could not pass the Russian, finishing in 52.77.
Hejnova Zuzana of Czech Republic won bronze in the event with a time of 53.38.