Heartland Health Region says a woman died in late June as a result of complications from the virus.
It's the same health region where a man recently died from the illness, but officials don't believe the deaths are related.
Hantavirus is rare and potentially fatal.
It is caused by exposure to airborne particles from the droppings or urine of infected rodents such as deer mice.
People can be exposed when sweeping or vacuuming out buildings such as cottages and sheds.
There have been 25 cases of hantavirus and eight deaths reported in Saskatchewan since 1994.
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