The director of Central Washington University's Chimpanzee and Human Communications Institute accompanied Tatu and Loulis to the Fauna Foundation, an 80-hectare sanctuary in Quebec.
The university says a primate veterinarian also flew with the chimps to monitor their vital signs.
The animals were lightly sedated for the trip.
Tatu and Loulis, who learned to communicate via American Sign Language, will be gradually integrated with the 11 other chimpanzees at the sanctuary.
Gloria Grow, one of the caretakers at the Fauna Foundation, is an alumna of the CWU program, so the two chimpanzees can continue to communicate via sign language.