Dwight Anderson, 32, and Taj Smith, 29, made their first appearance in Regina provincial court Thursday morning.
They're charged in connection with the alleged beating of a man outside a club on the 2000 block of Dewdney Ave. in the city's warehouse area on Aug. 18.
Regina police said officers were sent to the scene at around 2:40 a.m. CST after getting a report that there was a group of people involved in or gathered around a fight.
When police arrived they found the 20-year-old man with injuries consistent with being in a physical altercation. He was taken to hospital with serious injuries.
Following an investigation, police arrested Anderson and Smith on Wednesday at around 6 p.m.
Anderson, a defensive back, signed with the Riders in February. Smith, also known as Tajiddin Muquadir Smith-Wilson, is a receiver who's in his second year with the team.
On Thursday morning, the Saskatchewan Roughriders sent out a press release saying they would not comment on the charges until the matter is resolved.
Following their brief court appearance, Anderson and Smith were told they will be released until their next court appearance in October.
They are required to keep the peace, abstain from alcohol and drugs and stay away from clubs and bars.