Nona Marvisa Gaye and Frankie Christian Gaye's suit is the latest salvo in a dispute over Thicke's hit and whether it copies elements of Gaye's song "Got to Give It Up."
Their lawsuit seeks to block Thicke and collaborators Pharrell and T.I. from using elements of their father's music in "Blurred Lines" or other songs.
Thicke has denied copying Gaye's song for "Blurred Lines," which has the longest streak this year atop the Billboard Hot 100 chart and has sold more than 6 million tracks so far.
Much of the lawsuit focuses on claims that EMI should have pursued a copyright infringement claim. It accuses the company's executives of using intimidation to try to stop the Gaye family from pursuing a lawsuit.
The suit claims EMI, which is owned by Sony Music Entertainment, has allowed a conflict of interest between the family's rights and the profits it is earning from "Blurred Lines" sales.
"This conflict has resulted in EMI's intentional decision to align themselves with the ('Blurred Lines') writers, without regard to the harm inflicted upon the rights and interests of the Gaye Family, and the legacy of Marvin Gaye," the lawsuit states.
A phone message seeking comment from Sony Music was not immediately returned.
Thicke and his collaborators filed a case in August asking a federal judge to rule that the singers did not copy "Got to Give It Up" for their hit.
Howard King, who represents the singers, said the Gayes' countersuit was not unexpected, but he said their decision to sue EMI demonstrates the family lacks the appropriate authority to pursue the case against his clients.
He rejected the notion that EMI turned a blind eye to improper copying of Gaye's music. "EMI is in the business of collecting money for infringements," King said.
The company likely consulted a musicologist who found nothing improper, the attorney said. King said his firm consulted three music experts who determined the notes in the two songs were different.
Gaye's son Marvin Gaye III also might pursue legal action over the song, but he is not included in the federal court suit filed Wednesday.
Anthony McCartney can be reached at http://twitter.com/mccartneyAP