Paramedics said the woman gave birth to a baby boy while the plane was still in the air.
Earl Blacklock, who is with the territory's Department of Transportation, said there were multiple doctors and nurses aboard the flight who helped deliver the baby.
The mother and newborn were taken to Stanton Territorial Hospital by ambulance.
Airport officials said the KLM flight originated in Amsterdam and was en route to Calgary.
The plane landed in Yellowknife at 1 p.m. local time. The remaining passengers stayed on board, and officials said the flight resumed at 2:45 p.m.
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