Pirates general manager Neal Huntington had no additional details, but he said in a statement the team was "shocked" and "saddened" by Chambers' passing.
A third-round draft pick in 2009, Chambers spent five years in the team's minor league system. The outfielder moved up to Double-A Altoona in 2012. Chambers spent most of the 2013 season dealing with a foot injury and played in just four games for the team's Gulf Coast League affiliate as part of a rehab assignment.
Chambers was born in Miami and played high school baseball in Lakeland, Fla.
Several Pirates expressed their condolences on Twitter late Monday, with National League MVP Andrew McCutchen calling Chambers' death "a tough pill to swallow."