Humphries and Moyse laid down a track-record time of 56.63 seconds in the first heat to grab the lead, and edged Elana Meyers and Lauryn Williams of the U.S. by 0.12 seconds.
"To come out to any track and set a record, knowing no one's gone faster than that, it just proves to both of us and it cements that we're where we need to be," Humphries said.
Humphries and Moyse finished in one minute 53.66 seconds. Meyers and Williams were timed in 1:53.78, and Jamie Greubel and Katie Eberling finished third for the U.S. in 1:54.00.
Moyse, who's been nursing a back injury, did not train all week.
"She didn't push all week and then today comes in and we have the two fastest starts," Humphries said. "I think it shows the comfort we both have for each other."
Humprhies bounced back from her worst World Cup result in nearly two years, when her historic streak of 15 straight podium finishes — including 11 victories — ended in Park City, Utah last week. With Moyse out, heavy snow bounced Humphries and Chelsea Valois off the podium.
Edmonton's Jenny Ciochetti and Valois, of Zenon Park, Sask., placed 14th at 1:55.34.
Americans swept the podium in the men's event with Steven Holcomb adding to his undefeated start.
Holcomb and Chris Fogt finished two runs in 1:50.19. Nick Cunningham and Johnny Quinn were second in 1:50.74, and Cory Butner and Chuck Berkeley were third in 1:50.85 — capping off a sweep that came on the heels of the U.S. women winning a gold and tying for silver at a World Cup race in Park City, Utah last weekend.
Holcomb has now won all six World Cup men's bobsled races this season, four of them coming in two-man. He extended his lead in the World Cup two-man standings to 136 points over Cunningham.
But Holcomb was more impressed with the 1-2-3 U.S. finish than extending his own personal start.
"That's huge," Holcomb said. "These guys have been performing well all year. For them to finally put it together on the last day of 2-man, last day of the North American tour, I think that's really going to help bring that motivation and confidence into Europe. It's definitely going to be harder over there. We had to take advantage of our North American experience and capitalize on it here because trust me, it's going to get a lot more difficult."
Calgary's Chris Spring and Jesse Lumsden, of Burlington, Ont., were the top Canadian sled in sixth with a time of 1:51.02.
Justin Kripps, of Summerland, B.C., and Edmonton's Bryan Barnett posted their second-straight eighth-place finish with a time of 1:51.13.
"I'm really happy with the way we've started off the season," said Kripps. "I know we're capable of more. We have some things to clean up, but we are moving in the right direction."
Lyndon Rush, of Humboldt, Sask., and Calgary's Lascelles Brown were 13th at 1:51.77.
— With files from The Canadian Press