The Los Angeles Lakers shooting guard has suffered a fracture of the lateral tibial plateau in his left knee and is expected to miss six weeks, the team announced Thursday.
Bryant, 35, returned to the lineup 11 days ago, in a loss to the Toronto Raptors, after rupturing his Achilles on April 12.
“You hate it for Kobe. He’s worked so hard to get back," Lakers coach Mike D’Antoni was quoted as saying on the Lakers' Twitter account.
The team said the injury occurred when Bryant hyperextended his knee while backing down Tony Allen of the Memphis Grizzlies during a game on Tuesday.
Bryant, considered a surefire future hall of famer, signed a two-year, $48.5-million US contract extension at the end of November.
Bryant is averaging 13.8 points, 4.3 rebounds and 6.3 assists per game on the season.