Clayton Bo Eichler, 32, was to appear in court Friday charged with first-degree murder in the death of Richele Lee Bear, 23.
Eichler has also been charged with offering an indignity to a human body in the case.
Bear was last seen on Aug. 24, 2013.
Police said they conducted an extensive search, but her body has never been found. Investigators believe she is dead.
Eichler was previously charged with second-degree murder in the death of Kelly Nicole Goforth, 21.
Her body was found in the back alley of the 1700 block of McAra St., east of Regina's downtown, on the evening of Sept. 25, 2013.
Eichler was charged in her death on New Year's Eve.
Police said the charge in the Goforth case will be upgraded today to first-degree murder.
Members of Goforth's family were at the courthouse Friday to observe the proceedings.