Bautista, who will start in right field for the American League team at the July 15 all-star game at Target Field, received 5,859,019 votes to earn his fourth consecutive fan election and fifth overall all-star selection.
He also led the vote total in 2011, when his 7,454,753 votes broke the record previously set by Ken Griffey Jr. in 1994.
Bautista is one of three Blue Jays to be named to the AL all-star team, with first baseman Edwin Encarnacion and left-hander Mark Buehrle also making the cut.
MLB announced the rosters on Sunday.
Bautista, the captain of the AL squad at the 2014 Home Run Derby, leads the AL with a .423 on-base percentage and 60 walks, is seventh with 56 runs scored, eighth with a .529 slugging percentage and tied for 10th with 17 home runs.
Encarnacion, who injured his right quadriceps in Saturday's game against the Oakland Athletics, was chosen by AL all-star team manager John Farrell as a reserve. Encarnacion has 26 home runs and 70 RBIs through 88 games.
Buehrle was selected through the player ballot. The 35-year-old is 10-6 with a 2.60 ERA.