Police, fire and the Ministry of Transportation (MTO) are all responding to the collision, which has closed the lanes, said Const. Julia McCuaig. The driver received minor injuries.
A heavy tow truck is also on scene. Police have no estimate of how long the lanes will be closed.
Officers are still investigating how the collision happened, McCuaig said. Traffic is being redirected onto Lakeshore Road. The Fort Erie-bound lanes are open.
The province is in the midst of a $20-million construction project on the Skyway. Workers are on the second phase of applying anti-corrosive coating to the steel, which should be finished by fall 2016.
MTO is assessing the damage to the bridge.