In the audio, Adnani rallies fighters against the United States and the coalition it is leading in Iraq and Syria.
The speech, entitled, "Indeed, You Lord is Ever Watchful," came in a 41-minute, 44-second audio that was distributed with an English translation on Twitter on Sunday.
Directing a message to ISIS fighters, Adnani declared the military intervention by the U.S.-led coalition will be the "final campaign of the crusaders."
"It will be broken and defeated, just as all your previous campaigns were broken and defeated, except that this time we will raid you thereafter, and you will never raid us," Adnani says. "We will conquer your Rome, break your crosses, and enslave your women, by the permission of Allah, the Exalted. This is His promise to us; He is glorified and He does not fail in His promise.
"If we do not reach that time, then our children and grandchildren will reach it, and they will sell your sons as slaves at the slave market."
America, Adnani claimed, desires to wage a war on Islam, and he urged Muslims to take action and show their support for the ISIS through violence.
“If you can kill a disbelieving American or European — especially the spiteful and filthy French — or an Australian, or a Canadian, or any other disbeliever from the disbelievers waging war, including the citizens of the countries that entered into a coalition against the Islamic State, then rely upon Allah, and kill him in any manner or way however it may be.
"Do not ask for anyone's advice and do not seek anyone's verdict. Kill the disbeliever whether he is civilian or military, for they have the same ruling. Both of them are disbelievers. Both of them are considered to be waging war [the civilian by belonging to a state waging war against the Muslims]. Both of their blood and wealth is legal for you to destroy, for blood does not become illegal or legal to spill by the clothes being worn."
Again addressing the U.S. and its Western allies, 'Adnani promised retribution for their military intervention, and to bring the war to their soil.
“O Americans, and O Europeans, the Islamic State did not initiate a war against you, as your governments and media try to make you believe. It is you who started the transgression against us, and thus you deserve blame and you will pay a great price.
"You will pay the price when your economies collapse. You will pay the price when your sons are sent to wage war against us and they return to you as disabled amputees, or inside coffins, or mentally ill. You will pay the price as you are afraid of travelling to any land," 'Adnani says.
"Rather you will pay the price as you walk on your streets, turning right and left, fearing the Muslims. You will not feel secure even in your bedrooms. You will pay the price when this crusade of yours collapses, and thereafter we will strike you in your homeland, and you will never be able to harm anyone afterwards. You will pay the price, and we have prepared for you what will pain you.”