Two transport trucks and an SUV collided at about 2 a.m. in a construction zone near the Anciens Combattants exit.
According to police, the SUV was travelling between the two transport trucks when it was hit from behind.
The driver of the SUV was seriously hurt and transported to hospital.
Both of the transport truck drivers also suffered minor injuries.
Provincial police were on the scene Monday morning. The cause of the crash was not immediately clear.
Highway 40 was initially closed to eastbound traffic earlier, but has since been re-opened.